Skip to main content

Volume 16 Supplement 3

Sepsis 2012

Monocytic and neutrophilic CD11b and CD64 in severe sepsis

Background

Leukocyte immunophenotyping could improve sepsis diagnostics [1, 2]. Our hypothesis was that monocytic and neutrophilic CD11b and CD64 antigen fluorescence intensities differ between severe sepsis, non-inflammatory ICU patients and nonseptic inflammation (off-pump coronary artery bypass (OPCABG)).

Methods

Monocytic and neutrophilic CD11b and CD64 expressions were analyzed from 27 patients with severe sepsis, seven OPCABG patients and from eight ICU patients who did not fulfill any SIRS criteria. Blood samples were collected within 48 hours from the beginning of severe sepsis or in non-SIRS patients from ICU admission and two consecutive days (D0, D1, D2). From surgical patients, the first samples were taken on the day of surgery before the skin incision and two consecutive days (D0, D1, D2). In addition 10 healthy individuals served as controls. Samples were collected, processed and analyzed using flow cytometry as previously described [3].

Results

The maximum fluorescence intensities of monocytic and neutrophilic CD11b and CD64 were highest in septic patients compared with the other groups (P < 0.05) (Figure 1). In severe sepsis, fluorescence intensities decreased over time (P < 0.05). In OPCABG the fluorescence intensities of other antigens increased from D0 to D1 except neutrophilic CD11b (P < 0.05). The intensities of other antigens except neutrophilic CD64 were lower in the healthy than in all the other groups (P < 0.05). Neutrophilic CD64, as well as other antigens, were lower in healthy controls compared with severe sepsis at all time points (P < 0.05).

Figure 1
figure 1

Antigen fluorescence intensities in four groups in 3 days.

Conclusion

Based on this study, monocytic CD11b and neutrophilic CD64 could be helpful in distinguishing severe sepsis from nonseptic inflammation and healthy controls.

References

  1. Hoffmann J: Neutrophil CD64: A diagnostic marker for infection and sepsis. Clin Chem Lab Med 2009, 47: 903-916.

    Article  CAS  PubMed  Google Scholar 

  2. Nuutila J, Jalava-Karvinen P, Hohenthal U, Laitinen I, Kotilainen P, Rajamaki A, Nikoskelainen J, Lilius E: CRP/CD11b ratio: a novel parameter for detecting gram-positive sepsis. Hum Immunol 2009, 70: 237-243. 10.1016/j.humimm.2009.01.009

    Article  CAS  PubMed  Google Scholar 

  3. Jamsa J, Huotari V, Savolainen E-R, Syrjala H, Ala-Kokko T: Analysis of the temperature affects on leukocyte surface antigen expression. J Clin Lab Anal 2011, 25: 118-125. 10.1002/jcla.20444

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Cite this article

Jämsä, J., Huotari, V., Savolainen, E. et al. Monocytic and neutrophilic CD11b and CD64 in severe sepsis. Crit Care 16 (Suppl 3), P41 (2012). https://doi.org/10.1186/cc11728

Download citation

  • Published:

  • DOI: https://doi.org/10.1186/cc11728

Keywords

  • Fluorescence Intensity
  • Severe Sepsis
  • Artery Bypass
  • Coronary Artery Bypass
  • CD64 Expression