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Cerebral ischemia-reperfusion model in rabbits: relationship between dexmedetomidine and biochemical parameters in lowering intraparenchymal pressure
Critical Care volume 16, Article number: P328 (2012)
The effect of dexmedetomidine in two different doses on the levels of endothelin-1 (ET-1) and prostoglandin I2 (PGI2) in blood and cerebrospinal fluid (CSF) of rabbits via the transient global cerebral ischemia model was studied to determine its intraparenchymal pressure (IPP) reduction mechanism.
Twenty-four New Zealand type rabbits were employed and randomly distributed into four groups. Group I (sham group, n = 6): craniotomy was performed only. Group II (control group, n = 6): bilateral carotid arteries were clamped for 60 minutes after craniotomy, then reperfusion was performed for 60 minutes. In Group III (n = 6) and Group IV (n = 6), 80 μkg-1 and 320 μkg-1 dexmedetomidine was administered within the first 10 minutes of the reperfusion procedure respectively. Blood and CSF samples were collected 120 minutes after craniotomy. Mean arterial pressures (MAP), heart rates (HR), IPP and temperature values were recorded.
There was no significant difference in MAP values between groups (P ≥ 0.05). A decrease of HR in Group IV was significantly lower after reperfusion (P < 0.05). IPP values after the reperfusion in Groups II and IV were significantly higher than Group I (P < 0.05), but no significant increase in Group III (P ≥ 0.05). ET-1 levels of both blood and CSF were increased in the group with performed ischemia and reperfusion and no treatment (Group II) and the group administered high-dose dexmedetomidine (Group IV) (P < 0.05), while the group administered low-dose dexmedetomidine (Group III) was similar to the sham group (P ≥ 0.05). However, PGI2 levels of CSF were significantly decreased in the group administered low-dose dexmedetomidine (P < 0.05).
Zornow MH, Scheller MS, Sheehan PB: Intracranial pressure effects of dexmedetomidine in rabbits. Anesth Analg 1992, 75: 232-237.
Jolkkonen J, Puurunen K, Koistinaho J, et al.: Neuroprotection by the alpha 2-adrenoceptor agonist, dexmedetomidine in rat focal cerebral ischemia. Eur J Pharmacol 1999, 7: 31-36.
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Tavlan, A., Ustun, M., Yosunkaya, A. et al. Cerebral ischemia-reperfusion model in rabbits: relationship between dexmedetomidine and biochemical parameters in lowering intraparenchymal pressure. Crit Care 16, P328 (2012). https://doi.org/10.1186/cc10935
- Mean Arterial Pressure
- Sham Group
- Bilateral Carotid Artery