Skip to main content

Insufficient autophagy relates to mitochondrial dysfunction, organ failure and adverse outcome in an animal model of critical illness


Increasing evidence implicates mitochondrial dysfunction in the pathogenesis of critical illness-induced multiple organ failure. We previously demonstrated that prevention of hyperglycemia limits mitochondrial damage in vital organs [1, 2], thereby reducing morbidity and mortality [3]. We now hypothesize that inadequate activation of mitochondrial repair processes (mitochondrial clearance by autophagy, mitochondrial fusion and fission, and biogenesis) may contribute to accumulation of mitochondrial damage, persistence of organ failure and adverse outcome of critical illness.


We addressed this hypothesis in a rabbit model of critical illness. First, we studied whether vital organ mitochondrial repair pathways are differentially affected in surviving and nonsurviving hyperglycemic animals, in relation to mitochondrial and organ function. Next, we investigated whether preventing hyperglycemia with insulin affects mitochondrial repair over time. We quantified mRNA/protein levels of key players of these processes. Activities of respiratory chain complexes I to V were measured spectrophotometrically. Plasma transaminases and creatinine were measured as markers of liver, respectively kidney, dysfunction.


In the liver and kidney of nonsurviving hyperglycemic rabbits, molecular markers of insufficient autophagy were evident, including accumulation of p62 protein (but no increase of p62 mRNA) and decreases in the autophagosome-associated protein LC3-II (microtubule-associated protein light chain 3). These changes were less prominent in surviving animals and correlated with impaired mitochondrial and organ function. In contrast, key players in mitochondrial fusion, fission or biogenesis were not affected by survival status. Therefore, we focused on autophagy to study the impact of preventing hyperglycemia. Both after 3 and 7 days of illness, autophagy was better preserved in normoglycemic than in hyperglycemic rabbits, which correlated strongly with improved mitochondrial and organ function.


These findings put forward insufficient autophagy as a potentially important contributor to mitochondrial and organ dysfunction in critical illness, and open perspectives for therapies that activate autophagy during critical illness.


  1. Vanhorebeek I, et al.: Lancet. 2005, 365: 53-59. 10.1016/S0140-6736(04)17665-4

    CAS  Article  PubMed  Google Scholar 

  2. Vanhorebeek I, et al.: Kidney Int. 2009, 76: 512-520. 10.1038/ki.2009.217

    CAS  Article  PubMed  Google Scholar 

  3. Van den Berghe G, et al.: N Engl J Med. 2001, 345: 1359-1367. 10.1056/NEJMoa011300

    CAS  Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Cite this article

Gunst, J., Derese, I., Aertgeerts, A. et al. Insufficient autophagy relates to mitochondrial dysfunction, organ failure and adverse outcome in an animal model of critical illness. Crit Care 16 (Suppl 1), P11 (2012).

Download citation

  • Published:

  • DOI:


  • Hyperglycemia
  • Organ Failure
  • Critical Illness
  • Organ Function
  • Vital Organ