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Apoptosis of neutrophils, expression of TREM-1 on neutrophils and IL-17 responses in experimental burn in injury are related to the type and time of burn exposure

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Introduction

To define inflammatory responses in experimental burn injury in relation with the type and time of burn exposure.

Methods

Burn injury was induced in 110 C57/B6 male mice after time exposure of their back as follows: group 0, sham; group A, 60°C for 60 seconds; group B, 60°C for 45 seconds and 4°C for 45 seconds; group C, 75°C for 60 seconds; group D, 90°C for 5 seconds; and group E, 4°C for 45 seconds and 60°C for 45 seconds. Mice were sacrificed at 24 and 48 hours. Tissues were cultured and splenocytes were isolated and stimulated with heat-killed Staphylococcus aureus and Candida albicans for 5 days for release of IL-17. Neutrophil apoptosis and expression of TREM-1 were determined after staining for ANNEXIN-V, PI and anti-TREM-1-PE and flow cytometry analysis.

Results

Mean respective apoptosis of groups 0, A, B, C, D and E at 24 hours were 37.9%, 77.6%, 81.9%, 73.8%, 83.6% and 75.4%; and at 48 hours 78.5%, 79.4%, 77.7%, 78.2%, 81% and 84.9% (P < 0.05 group 0 vs. others). Mean respective MFI of TREM-1 of groups 0, A, B, C, D and E at 24 hours were 2.4, 4.4, 3.4, 3, 3.2 and 3; and at 48 hours 2.7, 2.8, 2.8, 2.6, 2.8 and 2.7 (P < 0.05 group 0 vs. others). Tissue cultures were sterile. Release of IL-17 was greater by splenocytes of group D (Figure 1).

Figure 1
figure1

Release of IL-17 by mice splenocytes in relation to the type of thermal injury.

Conclusion

Increased neutrophil apoptosis and TREM-1 expression and modulated IL-17 responses are found within burn injury.

Author information

Correspondence to A Alexis.

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Alexis, A., Carrer, D., Pistiki, A. et al. Apoptosis of neutrophils, expression of TREM-1 on neutrophils and IL-17 responses in experimental burn in injury are related to the type and time of burn exposure. Crit Care 16, P10 (2012). https://doi.org/10.1186/cc10617

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Keywords

  • Public Health
  • Flow Cytometry
  • Inflammatory Response
  • Tissue Culture
  • Time Exposure