- Poster presentation
- Open Access
Effect of low-dose steroid on NF-κB and caspase-3 intestinal expression in a sepsis mouse model
© Guntur et al. 2011
- Published: 27 October 2011
- Proinflammatory Cytokine
- Intestinal Tract
- Effector Caspase
- Acute Inflammatory Response
- Sepsis Group
The use of low-dose corticosteroids in sepsis early stages is still debated. The association of LPS-LBP complexes to CD14 receptors and will interact with TLR4 to induce NF-κB as a signal and transcription of proinflammatory cytokines [1, 2]. Excessive production of inflammatory cytokines will cause activation of SIRS, especially in gut-associated lymphoid tissues , which induces metabolic changes leading to apoptosis network, MOF, septic shock and death [3–5]. Changes in apoptosis are mediated by caspases, including caspase-3 that acts as an effector caspase [6, 7]. Low-dose corticosteroids can inhibit the production of proinflammatory cytokines, production of inflammatory mediators, and lower adhesion of leukocytes to the endothelium .
The aim of this study was to analyse NF-κB and caspase-3 intestinal expression, and also survival from use of low-dose steroid in the early stages of sepsis in the Balb/C mouse model of sepsis.
Male Balb/C mice were inoculated with lipopolysaccharide for the sepsis mouse model. Sepsis mouse model grouping was to a sepsis group (Group I) and to sepsis with steroid (methylprednisolone 1 to 1.5 mg/kg BW/day) (Group II). Detection of intestinal NF-κB and caspase-3 expression used the immunohistochemistry technique on days 1, 3, 5 and 7. Survival was seen until the 7th day. The two-tailed Fisher exact test for the analysis of mortality, independent-sample t test for intestinal NF-κB and caspase-3 expression, and P < 0.05 were used to determine significant differences.
NF-κB intestinal expression
Amount of cell expression
17.5 ± 4.7
10.8 ± 3.6
26.5 ± 4.4
15.8 ± 3.6
39.3 ± 4.1
31.2 ± 7.0
54.8 ± 9.6
50.5 ± 10.7
Caspase-3 intestinal expression
Amount of cell expression
8.5 ± 2.9
4.3 ± 1.9
14.7 ± 3.1
9.8 ± 2.2
33.2 ± 8.3
12.8 ± 4.5
42.3 ± 3.2
37.7 ± 8.2
Low-dose steroids can reduce NF-κB and caspase-3 intestinal expression and also mortality in early sepsis.
The authors thank the Faculty of Medicine, University of Sebelas Maret for their financial support and assistance given in this study.
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