- Poster presentation
- Open Access
Anti-endotoxin immunity in abdominal sepsis patients
- Published: 27 October 2011
Keywords
- Peritonitis
- Appendicitis
- Duodenal Ulcer
- Systemic Inflammatory Response Syndrome
- Humoral Immunity
Introduction
Anti-endotoxin immunity (AEI) has many biological effects but the problem of conjugation and elimination of lipopolysaccharides (LPS) in peritonitis patients is not discussed. We investigated the role of IgA, IgM and IgG in peritonitis and their association with humoral immunity (HI).
Methods
We investigated 33 patients (male:female = 25:8) with abdominal sepsis (total peritonitis in appendicitis, perforated duodenal ulcer, pancreonecrosis). Anti-endotoxin (AE) antibodies (anti-LPS-IgA, anti-LPS-IgM, anti-LPS-IgG) were determined by original modification of hard-phase immunoenzyme analysis. Escherichia coli K30 LPS was used as antigen for AE antibody detection. The level of general immunoglobulin was determined by the microturbidimetric method with human monospecific sera to IgG, IgA and IgM. All data were compared with healthy donors (99 patients).
Results
Table 1
AEI | HI | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
IgA | IgG | IgM | IgA | IgG | IgM | |||||||
Before surgery | After surgery | Before surgery | After surgery | Before surgery | After surgery | Before surgery | After surgery | Before surgery | After surgery | Before surgery | After surgery | |
Peritonitis patients | 0.28 ± 0.01, P < 0.05 | 0.45 ± 0.02 | 0.12 ± 0.01, P < 0/01 | 0.13 ± 0.02 | 0.21 ± 0.03, P < 0/05 | 0.29 ± 0.04 | 2.26 ± 0.16, P > 0.5 | 2.77 ± 0.18 | 10.1 ± 0.47, P > 0.5 | 10.98 ± 0.5 | 1.39 ± 0.11, P < 0.05 | 1.56 ± 0.12 |
Donors | 0.35 ± 0.05 | 0.16 ± 0.01 | 0.33 ± 0.05 | 2.21 ± 0.08 | 10.54 ± 0.242 | 1.66 ± 0.06 |
Conclusion
Abdominal sepsis patients are determined dysfunction of AEI (decrease of AE IgM and IgG). Successful treatment of peritonitis is accompanied with normalization of the IgM and IgG concentration and an increase of IgA above standard. Dynamics of AE antibodies may be a marker of the clinical course and forecast of abdominal sepsis. Comparative analysis of HI and AEI demonstrates parallelism of the dynamic concentration of immunoglobulins during treatment.
Authors’ Affiliations
Copyright
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.