Skip to main content

Exercise training reduces oxidative damage in skeletal muscle of septic rats

Introduction

Septic patients frequently develop critical illness myopathies (CIMs) that may represent a crucial factor for prolonged intensive care unit treatment and for ventilator weaning delay. Experimental findings have identified that oxidative stress plays a role in causing muscle depletion in chronic pathological states like sepsis. It is well documented that regular moderate physical exercise can decreased oxidative stress and enhance antioxidant functions.

Objective

To investigate whether exercise training reduces oxidative damage in septic rats induced by cecal ligation and perforation (CLP).

Methods

Wistar rats were randomly assigned to three groups: Sham (submitted to a fake surgery), CLP, and CLP that was previously trained (CLPT). The exercise training protocol consisted of 8 weeks of running on a treadmill, 5 days/week, for 60 minutes at 60% of the maximal running speed obtained on the graded treadmill test. Rats were subjected to CLP surgery; after 120 hours of surgical procedure they were killed by decapitation. Oxidative damage of lipids (thiobarbituric acid reactive species (TBARS)) and proteins (carbonyl groups) were analyzed in Soleus (type I fiber) and plantaris (type II fiber) muscles.

Results

See Table 1

Table 1 Levels of TBARS and carbonyl of soleus and plantaris muscles

Conclusion

TBARS and carbonyl analysis for CLPT are lower than for CLP with statistical significance, except for carbonyl plantaris with P = 0.06 (Table 1 and Figure 1). Our data supported that exercise training before sepsis could decrease oxidative damage in both muscle fiber types.

Figure 1
figure1

Levels of TBARS of soleus (a) and plantaris (b); and levels of protein carbonyl of soleus (c) and plantaris (d). Values presented as mean ± SEM. *Significant difference in relation to Sham group (P < 0.05); #Significant difference in relation to CLP group (P < 0.05).

Author information

Affiliations

Authors

Corresponding author

Correspondence to CW Coelho.

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Cite this article

Coelho, C., Jannig, P., Souza, A. et al. Exercise training reduces oxidative damage in skeletal muscle of septic rats. Crit Care 15, P2 (2011). https://doi.org/10.1186/cc10150

Download citation

Keywords

  • Oxidative Damage
  • Exercise Training
  • Myopathy
  • Thiobarbituric Acid
  • Septic Patient