Fig. 6

APOH decreases inflammatory cytokine responses in LPS-treated macrophage cells. a The mRNA expression levels of IL-1β and TNF-α in PMs were determined by qPCR. b–c Western blot analysis of the relative expression levels of IL-1β and TNF-α in PMs. d The mRNA expression levels of IL-1β and TNF-α in BMDMs were determined by qPCR. e–f Western blot analysis of the relative expression levels of IL-1β and TNF-α in BMDMs. g The mRNA expression levels of IL-1β and TNF-α in PBMCs were determined by qPCR. h–i Western blot analysis of the relative expression levels of IL-1β and TNF-α in PBMCs. j The mRNA expression levels of IL-1β and TNF-α in RAW 264.7 cells were determined by qPCR. k–l Western blot analysis of the relative expression levels of IL-1β and TNF-α in RAW 264.7 cells. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001. Apolipoprotein H, APOH; peritoneal macrophages, PMs; lipopolysaccharide, LPS; bone marrow-derived macrophages, BMDMs; peripheral blood mononuclear cell, PBMCs; interleukin, IL; tumour necrosis factor, TNF; Quantitative Real-time reverse transcriptase-polymerase chain reaction, qPCR