Fig. 4

Anti-APOH antibody worsens non-severe sepsis in mice. a Ligation of the caecum at designated positions to establish a non-severe CLP model. The caecum was ligated (indicated by the dotted green line) at a 40% distance between the distal pole and the base of the caecum (yellow dotted line). b The procedures for the administration of an anti-APOH antibody in mice with CLP-induced non-severe sepsis. c Survival of mice with CLP-induced non-severe sepsis after treatment with the anti-APOH antibody (n = 8). d Lung, liver, and kidney tissues were stained with H&E in the IgG control and anti-APOH groups. e Semiquantitative scores of tissues were calculated in the IgG control and anti-APOH groups (n = 7). f Serum cytokine levels in blood in the IgG control and anti-APOH groups at 24 h after non-severe CLP (n = 7). g Cytokine levels in the PLF in the IgG control and anti-APOH groups at 24 h after non-severe CLP (n = 7). The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001. Apolipoprotein H, APOH; caecal ligation and puncture, CLP; peritoneal lavage fluid, PLF; haematoxylin and eosin staining, H&E