Fig. 3

Administration of recombinant APOH protein protects septic mice. a Ligation of the caecum at designated positions to establish a severe CLP model. The caecum was ligated (indicated by the dotted green line) at a 60% distance between the distal pole and the base of the caecum (yellow dotted line). b The procedures for rAPOH treatment in CLP-induced severe sepsis. c Survival of mice with CLP after treatment with BSA and rAPOH (n = 8). d Lung, liver, and kidney tissues were stained with H&E in the BSA and rAPOH groups. e Semiquantitative scores of tissues were calculated in the BSA and rAPOH groups (n = 7). (f) Serum cytokine levels in the BSA and rAPOH groups at 24 h after severe CLP (n = 7). g Cytokine levels in the PLF in the BSA and rAPOH groups at 24 h after severe CLP (n = 7). The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Apolipoprotein H, APOH; recombinant murine APOH, rAPOH; bovine serum albumin, BSA; caecal ligation and puncture, CLP; peritoneal lavage fluid, PLF; haematoxylin and eosin staining, H&E