Fig. 6

Interleukin (IL)-36R are increased in lung fibroblasts and epithelial cells. A Fibroblasts were purified from lung of wild type mice after CLP 3 days. Gene expression for interleukin (IL)-36R was analyzed by quantitative PCR. B Epithelial cells were purified from lung of wild type mice after CLP 3 days. Gene expression for interleukin (IL)-36R was analyzed by quantitative PCR. C Sections from lung fibroblasts in CLP-induced mice were analyzed for IL-36R by immunofluorescence (IF). Arrows indicate IL-36R expression. D Sections from lung epithelial cells in CLP-induced mice were analyzed for IL-36R by immunofluorescence (IF). Arrows indicate IL-36R expression. E Fibroblasts were purified from lung of wild type and IL-36R knock out mice after CLP 3 days. Gene expression for lipocalin 2 (LCN2) was analyzed by quantitative PCR. F Sections from lung fibroblasts in CLP-induced wild type and IL-36R knock out mice were analyzed for LCN2 by immunofluorescence (IF). Arrows indicate LCN2 expression. Three independent experiments were performed thrice. Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test). Data are means ± SD and error bars represent SD. P ≤ 0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001