Fig. 5

IL-36R deficiency in nonhematopoietic cells accounted for the enhanced severity to CLP-induced sepsis. A Scheme of BM chimeric mice construction and experimental design. B Survival curves of mice from the four groups of BM chimeric mice treated with CLP (n = 14 to 16 mice per group) were recorded over a 7-day period. Comparison between groups was done by Kaplan–Meier analysis followed by log–rank tests. C Serological markers of organ injury including alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine in CLP-induced BM chimeric mice at 3 days after CLP. Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test). D Lung homogenates from CLP-induced BM chimeric mice were cultured on blood agar plates, and the number of bacterial colonies was counted as colony-forming unit (CFU). Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test). E Representative flow cytometry plots showing the frequency of neutrophils(Ly6G+), in lung tissue from CLP-induced BM chimeric mice. F Pooled flow cytometry data. G The histological images of H&E stained lung sections from CLP-induced BM chimeric mice; the scale Bar = 100 μm. H Total lung injury score obtained from 5 pathophysiological characteristics based on the histological images. Three independent experiments were performed thrice. Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test). Data are means ± SD and error bars represent SD. P ≤ 0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001