Fig. 3

Interleukin (IL)-36R-signalling afforded protection against sepsis. A Survival of cecal ligation and puncture (CLP) and sham operation mice (n = 16 per group) after interleukin (IL)-36R knock out or not. Comparison between groups was done by Kaplan–Meier analysis followed by log-rank tests. B Serological markers of organ injury including alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine in interleukin (IL)-36R knock out mice at 3 days after CLP. Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test). C Vital organs homogenates from CLP mice with or without interleukin (IL)-36R knock out were cultured on blood agar plates, and the number of bacterial colonies was counted as colony-forming unit (CFU). Statistical difference was denoted by the horizontal bracket (Mann–Whitney U test). Three independent experiments were performed thrice. Data are means ± SD and error bars represent SD. P ≤ 0.05 were considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001