Fig. 3

NIR limits post-ischemic mitochondrial swelling and reduces mitophagy in primary neurons. A Experimental approach B Representative images of MitoQC (GFP) for machine learning classification. Inserts: binary maps of mitochondrial objects labeled by morphological classification (networks: green, unbranched: blue, red: swollen, magenta: punctate). C Mitochondrial network analysis following OGD + 2 h, 4 h and 6 h of reoxygenation. Mitochondrial network analysis with and without NIR therapy. (n = 6 per group, *p < 0.05, **p < .01, ***p < 0.001, by two-way ANOVA and post hoc Tukey test, mean + SD). D Primary neurons extracted from MitoQC mice show increased mitophagy following OGD (red signal). E Mitophagy is significantly reduced with NIR therapy. (n = 5 per group, *p < 0.05, **p < 0.01, by two-way ANOVA post hoc Tukey, data represented as Mean + SD)