Fig. 3

Patients homozygous for the major allele of rs9984273 (TT) have higher STAT2 expression in the lungs than the heterozygous patients with the minor allele (CT) and glucocorticoids do not inhibit its nuclear expression in TT patients. A STAT1 expression in the lung after a 4-day culture in the presence of IFN β with or without hydrocortisone (HC). B pSTAT1 expression in the same specimens as in A. C Example photomicrographs showing higher STAT2 expression in a TT patient than in a CT patient and the effect of HC on its nuclear translocation. Most STAT2 remains in the cytoplasm of the CT patients, whereas nuclear expression is prominent in the TT patient. Indicated insets are shown in the bottom row. D Combined results of all patients noting that two CT samples are excluded in the data as the patients were already under glucocorticoid treatment at the time of sample acquisition. E pSTAT2 expression in the same samples as in A, B and D. Ns, not significant; *P < 0.05; **P < 0.01; and ***P < 0.001. Statistics: two-way ANOVA with Šídák's multiple comparisons test