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Table 4 AMR genes detected by ddPCR and the related pathogens detected by blood culture and all microbiological testing

From: Clinical validation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections in ICU practice: a promising diagnostic tool

AMR genes

Pathogens

ddPCR+

n, (%)

BC+ and according to AST, n, (%) *

Microbiological testing and, according to AST n, (%)

blaKPC (n = 40)

klebsiella pneumoniae

30 (75.0)

6 (15.0)

40 (100)

 

None

10 (25.0)

34 (85.0)

0 (0)

blaNDM (n = 3)

None

3 (100)

3(100)

3(100)

mecA (n = 38)

Staphylococcus aureus

3 (7.9)

0

9 (23.7)

 

Staphylococcus haemolyticus

–

2 (5.3)

5 (13.2)

 

Staphylococcus capitis

–

2 (5.3)

2 (5.3)

 

Staphylococcus epidermidis

–

3 (7.9)

6 (15.8)

 

Staphylococcus hominis

–

1 (2.6)

1 (2.6)

 

Staphylococcus simulans

–

1 (2.6)

1 (2.6)

 

Staphylococcus warneri

–

1 (2.6)

1 (2.6)

 

None

 

28 (73.7)

13 (34.2)

  1. *Staphylococcus haemolyticus and Staphylococcus epidermidis were detected by blood culture in one sample. AMR gene, antimicrobial resistance gene; CRKP, carbapenem-resistant Klebsiella pneumoniae