Fig. 5

SARS-CoV-2 spike RBD protein activated the NOX1 and NOX2. Mice were exposed to LPS with or without SARS-CoV-2 spike RBD-Fc protein for 3 days. A The mRNA expression levels of AT₁R and AT₂R in the lung tissues of mice with indicated treatment were determined by qPCR analysis (n = 5 per group). B The protein expression levels of IκB and p-IκB in the lung tissues were determined by western blot analysis, and the representative results were shown. C, D The mRNA expression levels of indicated gene in the lung tissues were determined by qPCR analysis (n = 5 per group). E The protein expression levels of NOX1 and NOX2 in the lung tissues were determined by western blot analysis, and the representative results were shown. Data are shown as mean ± S.D., *P < 0.05, **P < 0.01, and N.S. indicates not significant