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Fig. 3 | Critical Care

Fig. 3

From: Exosomal miR-30d-5p of neutrophils induces M1 macrophage polarization and primes macrophage pyroptosis in sepsis-related acute lung injury

Fig. 3

miRNA analysis of PMN-derived exosomes. a Heat map of exosomal miRNA-seq (n = 3). The fluorescence intensity of 26 differentially expressed miRNAs (≥ twofold) is illustrated from high (red) to low (blue). b Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis on differentially expressed exosomal miRNAs; the 20 most enriched pathways related to signaling transduction are shown. The rich ratio indicates the number of genes in the miRNA target list over the total genes in the respective canonical pathway. c–e Expression of miR-30d-5p in PMN-derived exosomes, in PMNs stimulated with PBS/TNF-α and in recipient Raw264.7 macrophages treated with PBS/PBS-Exo/TNF-Exo by RT-qPCR. Student’s t test (c, d) or one-way analysis of variance with Tukey's multiple comparisons test (E) was used for the analysis. Graphs represent means ± SEM, n ≥ 3; *P < 0.05, **P < 0.01 compared within two groups

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Critical Care

ISSN: 1364-8535