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Table 2 List of primers used for quantification of mRNA and DNA expression levels by qPCR and RT-qPCR

From: Sepsis is associated with mitochondrial DNA damage and a reduced mitochondrial mass in the kidney of patients with sepsis-AKI

Gene

Primer sequence

ND1, ETC complex 1

Forward:

TGGCTCCTTTAACCTCTCCA

Reverse:

GGTTCGGTTGGTCTCTGCTA

ND4, ETC complex 1

Forward:

GGCGGCTATGGTATAATACG

Reverse:

GTAGGCAGATGGAGCTTGTT

ND6, ETC complex 1

Forward:

TGATTGTTAGCGGTGTGGTC

Reverse:

CCTCAATAGCCATCGCTGTA

COX1, ETC complex 4

Forward:

CTAACAGACCGCAACCTCAA

Reverse:

CCGAAGCCTGGTAGGATAA

Cytochrome b, ETC complex 3

Forward:

TTCCTAGCCATGCACTACTC

Reverse:

GAAGGTAGCGGATGATTCAG

D-loop, regulative region

Forward:

AACCTACCCACCCTTAACAG

Reverse:

CACTCTTGTGCGGGATATTG

β2M

Forward:

CTGGGTAGCTCTAAACAATGTATTCA

Reverse:

CATGTACTAACAAATGTCTAAAATGGT

  1. ETC electron transport chain
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Critical Care

ISSN: 1364-8535