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Fig. 4 | Critical Care

Fig. 4

From: The circular RNA landscape in specific peripheral blood mononuclear cells of critically ill patients with sepsis

Fig. 4

Experimental validation of detected circRNAs in healthy CD14+ monocytes. a Agarose gel scan illustrating amplicons of linear mRNA (HPRT1) and two circRNAs VCAN (chr5: 83519349-83522309) and CHD2 (chr15: 93000512-93014909) treated with (+) or without (−) RNase R. Ladder, Thermo Scientific GeneRuler DNA Ladder mix #SM0333. b Quantitative RT-PCR normalized expression patterns of VCAN (chr5: 83519349-83522309) and CHD2 (chr15: 93000512-93014909) circRNAs in healthy monocytes. c Box plots depicting the production of TNF and IL-6 by monocytes stimulated with lipopolysaccharide (LPS), heat-killed Streptococcus pneumoniae (Sp), and Klebsiella pneumoniae (Kp) for 2 and 24 h. *p < 0.05. d Box plots illustrating the normalized expression patterns of circRNAs, circVCAN (chr5: 83519349-83522309) and circCHD2 (chr15: 93000512-93014909), in stimulated monocytes

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Critical Care

ISSN: 1364-8535