Fig. 3
From: Alterations of the iNKT cell compartment in brain-injured patients
Analysis of lymphocytes population in PBMC by flow cytometry. Box and whisker plot summarizing the number of lymphocytes in BI patients. The gray area shows the normal range in healthy population (1.5–4 × 109 cells/L) (a). After α-GalCer expansion, iNKT cell populations were compared. PBMCs from 10 healthy volunteers (HV) (white boxes) or PBMC from BI (gray boxes), 9 with pneumonia, and 8 without pneumonia were cultured in medium containing α-GalCer and IL-2 for 10 days to induce iNKT cell expansion. Boxes and whisker plots summarizing the percentage of iNKTs in the CD3+ compartment before stimulation (b) or after stimulation (c), the ratio of CD4+/CD4− iNKT cells (d), the percentage of iNKT IFN-γ+ (e), and the percentage of iNKT IL-4+ (f). Top and bottom whiskers represent the extreme values; boxed area represents the 25th percentile, the median, and the 75th percentile (**p < 0.01, ***p < 0.001; statistical analysis, Mann-Whitney test)