Fig. 2

Blocking lipolysis aggravated muscle wasting and weakness in overweight/obese septic mice. Markers of muscle wasting and weakness were assessed in overweight/obese (Ob) septic wild-type (WT) and adipose tissue-specific ATL knockout (AAKO) mice after 5 days of illness. a Tibialis anterior (TA) and b extensor digitorum longus (EDL) muscle dry weight. c Relative mRNA expression of muscle atrophy markers. Gene expression data are normalized to Rn18s and presented relative to the mean of WT Ob healthy controls (Ctrl). d Representative force tracings of ex vivo-measured absolute maximal tetanic force of the EDL muscle. e Ex vivo force measurements of the EDL indicating the specific maximal force (peak tetanic tension per unit muscle mass). a, b, c Ob Ctrl: WT n = 19, AAKO n = 18; Ob Sepsis: WT n = 19, AAKO n = 17. e Ob Ctrl: WT n = 17, AAKO n = 14; Ob Sepsis: WT n = 14, AAKO n = 14. Data are means ± SEM. p values determined through Wilcoxon Test [Wilcoxon p values: a p < 0.0001, b p < 0.0001, c Fbxo32 p < 0.0001, Trim63 p < 0.0001, e p < 0.0001]. § p ≤ 0.05, §§ p ≤ 0.01, §§§ p ≤ 0.001 between Ctrl and Sepsis and * p ≤ 0.05, ** p ≤ 0.01, ***p ≤ 0.001 between sepsis groups