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Fig. 5 | Critical Care

Fig. 5

From: The interleukin-27 -964A>G polymorphism enhances sepsis-induced inflammatory responses and confers susceptibility to the development of sepsis

Fig. 5

The IL-27 plasmid constructs and functional promoter activities of rs153109 in 293 T, THP-1 and HUVECs. The promoter region of IL-27 DNA sequence (2145 bp) carrying A or G of rs153109 A > G was cloned into pGL3 luciferase reporter vectors (a); the different IL-27 plasmid constructs were transfected into 293 T, THP-1 and HUVEC cells for 48 h, and then the promoter activities were detected by dual-luciferase report assays (b, c, d). Three parallel samples were used in all transfections, and all experiments were performed in triplicate. The error bar represents standard error of the mean

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Critical Care

ISSN: 1364-8535