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Fig. 3 | Critical Care

Fig. 3

From: 6% Hydroxyethyl starch (HES 130/0.4) diminishes glycocalyx degradation and decreases vascular permeability during systemic and pulmonary inflammation in mice

Fig. 3

Hydroxyethyl starch (HES) 130/0.4 effects on the thickness of the glycocalyx during systemic and pulmonary inflammation. a Schematic illustration of the fluorescein isothiocyanate (FITC) exclusion technique used to visualize the glycocalyx (ESL = endothelial surface layer) width in the cremaster and pulmonary microcirculation in vivo. Mice underwent cecal ligation and puncture (CLP) or a sham operation (b) or lipopolysaccharide (LPS) or saline inhalation (c), which was 1 h later followed by an infusion of 20 ml/kg Isolyte® (Iso) or HES 130/0.4 (HES) over a period of 1 h. At 4, 8, and 24 h after the operation or inhalation, respectively, the thickness of the glycocalyx was measured in the cremaster muscle in the CLP experiment and in the lung capillaries in the LPS inhalation experiment by intravital microscopy using the FITC-dextran exclusion technique. Data are presented as mean ± SEM, * p < 0.05

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Critical Care

ISSN: 1364-8535