Inflammasome and toll-like receptor signaling in human monocytes after successful cardiopulmonary resuscitation

Table 2 Monocyte mRNA expression in 30-day survivors and nonsurvivors after sudden cardiac arrest

mRNA RCN ± SD	CPR t1		P value	CPR t2		P value	CPR t3		P value
	Survivors (n = 11)	Nonsurvivors (n = 18)		Survivors (n = 11)	Nonsurvivors (n = 18)		Survivors (n = 12)	Nonsurvivors (n = 11)
TLR2	1.69 ± 1.00	2.16 ± 0.84	0.061	1.32 ± 0.92	1.15 ± 0.40	0.947	1.09 ± 0.41	0.68 ± 0.17	0.003
TLR4	0.40 ± 0.15	0.51 ± 0.15	0.055	0.32 ± 0.06	0.35 ± 0.12	0.740	0.35 ± 0.09	0.33 ± 0.08	0.525
IRAK3	0.70 ± 0.80	0.67 ± 0.23	0.055	0.44 ± 0.17	0.47 ± 0.18	0.611	0.61 ± 0.37	0.35 ± 0.08	0.027
IRAK4	0.29 ± 0.32	0.26 ± 0.07	0.089	0.26 ± 0.12	0.24 ± 0.09	0.521	0.30 ± 0.21	0.17 ± 0.03	0.027
NLRP1	0.48 ± 1.14	0.13 ± 0.06	0.774	0.20 ± 0.12	0.14 ± 0.06	0.134	0.26 ± 0.32	0.10 ± 0.03	0.059
NLRP3	0.53 ± 0.59	0.51 ± 0.30	0.412	0.42 ± 0.44	0.41 ± 0.52	0.877	0.42 ± 0.40	0.16 ± 0.06	0.006
AIM2	0.015 ± 0.02	0.006 ± 0.003	0.220	0.006 ± 0.003	0.006 ± 0.003	0.774	0.012 ± 0.006	0.013 ± 0.010	0.880
PYCARD	0.11 ± 0.07	0.13 ± 0.05	0.112	0.16 ± 0.05	0.17 ± 0.10	0.550	0.14 ± 0.10	0.09 ± 0.03	0.059
CASP1	0.81 ± 0.40	0.78 ± 0.24	0.912	0.82 ± 0.17	0.80 ± 0.41	0.363	0.88 ± 0.39	0.58 ± 0.11	0.019
IL1B	1.03 ± 2.67	0.50 ± 1.03	0.877	1.25 ± 3.12	0.79 ± 2.13	0.465	1.01 ± 2.49	0.04 ± 0.07	0.118

Shown are monocyte mRNA expression levels, expressed as mean relative copy numbers (RCN) ± standard deviation (SD), in 30-day survivors and nonsurvivors in the first 12 h (cardiopulmonary resuscitation (CPR) t1; n = 29), after 24 h (CPR t2; n = 29), and 48 h (CPR t3; n = 23) after CPR. There was one patient in group CPR t1 who was lost to follow-up after study enrollment. Statistical hypothesis testing was performed using the Mann–Whitney U test. TLR toll-like receptor, IRAK interleukin-1 receptor-associated kinase, NLRP NLR family pyrin domain containing, AIM absent in melanoma, PYCARD PYD and CARD domain containing, CASP caspase

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