Fig. 7

MFG-E8-derived short peptide 68 (MSP68) does not affect oxidative burst activity of neutrophils after cecal ligation and puncture (CLP). Peritoneal fluids from sham, vehicle and MSP68-treated mice were harvested at 20 h after CLP and processed to obtain single cell suspensions. Isolated peritoneal cells were incubated with 50 μM of dihydrorhodamine 123 (DHR) at 37 °C for 30 minutes, followed by staining with allophycocyanin (APC)-anti-mouse Ly-6G and peridinin chlorophyll protein-cyanine 5.5 (PerCP/Cy5.5)-anti-mouse CD11b. Flow cytometry was done to quantify the fluorescence from DHR. a Representative histogram overlays show the flow cytometric analysis of DHR on the gated peritoneal neutrophils. b The graphs show the spontaneous neutrophil oxidative burst activity as the mean fluorescence intensity (MFI) of DHR on gated peritoneal neutrophils. Data are expressed as mean ± standard error of the mean (n = 3/group) and compared by one-way analysis of variance and Student–Newman–Keuls test; *P <0.05 versus sham