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Figure 2 | Critical Care

Figure 2

From: Levosimendan affects oxidative and inflammatory pathways in the diaphragm of ventilated endotoxemic mice

Figure 2

Markers of nitrosative and oxidative stress. (A) Expression of inducible nitric oxide synthase (iNOS) in the diaphragm. iNOS expression was increased in mechanically ventilated endotoxemic mice (MV LPS) versus unventilated mice (CON) (*P <0.05). iNOS expression was increased in mechanically ventilated endotoxemic mice treated with levosimendan (MV LPS + L) versus CON and MV LPS (#P <0.05). GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; L, Levosimendan; LPS, Lipopolysaccharide. (B) Nitrotyrosine concentration in the diaphragm. Nitrotyrosine concentration was higher in MV LPS mice than in MV LPS + L mice (*P <0.05). OD, Optical density. (C) Representative nitrotyrosine blot. (D) 4-hydroxyl-2-nonenal (HNE) protein concentration relative to actin concentration in diaphragm. HNE protein concentration was higher in MV LPS than in MV LPS + L mice (*P <0.05). (E) Representative HNE protein blot.

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Critical Care

ISSN: 1364-8535