Figure 2

Calculated difference from reference (DFR) for 51 of the 63 known genes that distinguish clinical trajectory. Using a false discovery adjusted probability <0.001 and a twofold difference in expression, the temporal pattern of expression of the 51 genes that differed between the matched aged (≥55 years old) and young (<55 years old) trauma patients with complicated outcomes, as well as healthy controls, was analyzed and used to calculate a DFR score. The summary of the DFR scores for the patients in each cohort at days 0.5, 1.0 and 4.0 after traumatic injury is presented. Statistical analysis at 0.5, one and four days revealed significant differences in the DFRs between the young and aged. On days 0.5 and 1.0, the expression patterns in the young complicated trauma patients were significantly more aberrant from control to those seen in the advanced age cohort. By day 4, the expression patterns in the aged were found to be significantly more aberrant from controls than those seen in the young (Newman-Keuls multiple comparison test, * P <0.05).