Figure 3
From: Neutralization of osteopontin attenuates neutrophil migration in sepsis-induced acute lung injury
Effect of anti-OPN Ab on the expression of proinflammatory cytokines and chemokine in the lungs after CLP . Mice were subjected to CLP and 100 μl of anti-OPN Ab (50 μg/mouse) or PBS as vehicle was injected through the jugular vein. Lung tissues were collected after 20 h from sham-operated, vehicle, and anti-OPN Ab-treated mice. The tissue expression of (A) IL-6, (B) IL-1β and (C) MIP-2 was determined by real-time PCR. Gene expression was normalized to β-actin. The sham expression level was designated as one for comparison. Similarly, 50 μg of total protein extracted from the lung tissues were examined for (D) IL-6, (E) IL-1β and (F) MIP-2 analysis by ELISA. Finally, the results are expressed as per mg of proteins. Data are represented as means ± SEM (n = 5 mice/group) and compared by one-way ANOVA and SNK method (* P <0.05 vs. sham; # P <0.05 vs. vehicle). Ab, antibody; ANOVA, analysis of variance; CLP, cecal ligation and puncture; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; MIP-2, macrophage inflammatory protein 2; OPN, osteopontin; PBS, phosphate-buffered saline; PCR, polymerase chain reaction; SEM, standard error of the mean; SNK, Student-Newman-Keuls.