Increasing the inspiratory time and I:E ratio deteriorated lung function in VILI. Mice were mechanically ventilated for 4 hours with either low tidal volume (LVT 9 ml/kg) or high tidal volume (HVT 34 ml/kg) and an inspiratory/expiratory ratio of 1:2 or 1:1, respectively. An alternative end point was defined as decreasing of mean arterial blood pressure below 40 mm Hg, which predicts death with certainty in this model. Controls (ctr) were subjected to LVT 1:2 ventilation only during operation and were killed before the 4-hour ventilation protocol started. (A) Dynamic elastance (E) was measured every 10 minutes during the experiment. Results of end-point measurements of dynamic compliance (B) and static compliance (C) are shown. n = 13 to 14 in each group; ***P < 0.001.