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Table 1 Primers and PCR conditions

From: Rs1800625 in the receptor for advanced glycation end products gene predisposes to sepsis and multiple organ dysfunction syndrome in patients with major trauma

SNP

Primers for PCR

Sequencing primer

SNP sequence

Annealing temperature

rs1800625

F: *bio-TCTTTTTTCCCTGGGTTTAGTTGA

GAGAGAAACCTGTTTGG

AA/GCTTC

60Ā°C

R: ATAGGGTTCAGGCCAGACTGTTGT

  1. *biotin labeling.