The protective effects of a phosphodiesterase 5 inhibitor, sildenafil, on postresuscitation cardiac dysfunction of cardiac arrest: metabolic evidence from microdialysis

Table 3 The contents of ATP, ADP, phosphocreatine, Na ⁺ -K ⁺ -ATPase and Ca ²⁺ -ATPase activities in left ventricle tissue at 24 hours after successful resuscitation ^a

Group	Phosphocreatine (nmol/mg protein)	ATP (nmol/mg protein)	ADP (nmol/mg protein)	Na ⁺-K ⁺-ATPase (U)	Ca ²⁺-ATPase (U)
Sham	33.36 ± 7.27	16.49 ± 1.67	1.51 ± 0.12	9.21 ± 1.45	10.96 ± 0.94
SA	19.71 ± 9.41**	10.49 ± 1.08**	0.91 ± 0.10**	4.97 ± 1.04**	3.65 ± 1.01**
Sildenafil	26.43 ± 4.66*^†	12.3 ± 1.11*^†	1.11 ± 0.21*^†	6.89 ± 1.37*^†	4.58 ± 1.43**^†

^aValues are mean ± SD. SA, Saline. *P < 0.05 vs. sham, **P < 0.01 vs. sham, ^†P < 0.05 vs. SA. All calculations were performed using one-way repeated-measures analysis of variance. All posttests were performed using the Bonferroni method. Myocardial tissue analysis revealed a dramatic increase in the content of ATP, ADP and phosphocreatine in the sildenafil group compared with the SA group at 24 hours after ROSC (P = 0.03, P = 0.02, P = 0.02, respectively). The activities of left ventricle Na⁺-K⁺-ATPase and Ca²⁺-ATPase were significantly increased in the sildenafil group compared with the SA group at 24 hours after ROSC (P = 0.03, P = 0.04, respectively).

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