Figure 7

Trim62 is involved in atrophy signaling. (A) Luciferase assays were performed on cell extracts of HEK293 cells transfected with the activator protein 1 (AP-1) luciferase reporter plasmid, along with expression plasmids encoding the Trim62 protein (+) or empty (-) expression plasmid. Values were normalized to expression of CMV-LacZ and are expressed as the relative luciferase activity to CMV-LacZ ratio with the Trim62 expression plasmid compared to the reporter alone. Data are shown as mean ± SEM. *P <0.05. (B) Differentiated C2C12 myotubes were transfected with control scrambled small interfering RNA (siSCR) or siRNA against Trim62 (siTrim62) (25 nM each), as indicated. Cells were harvested 24 hours after transfection, and Trim62 expression was quantified using quantitative RT-PCR analysis. Glyceraldehyde-3 phosphate dehydrogenase (Gapdh) expression was used as the reference value. Data are presented as mean ± SEM. **P <0.01. (C) Differentiated C2C12 myotubes were transfected with siSCR or siTrim62 (25 nM each) and treated with vehicle (-) or lipopolysaccharide (LPS, +, 100 μm, for 2 hours), as indicated. RT-PCR was used to quantitate Trim62 expression. Gapdh expression was used as the reference value. Data are presented as mean ± SEM. *P <0.05. IL-6, Interleukin 6.