Figure 4

Neutralization of monocyte chemoattractant protein-1 (MCP-1) reduces myocardial mononuclear cell accumulation and cytokine production in old mice. MCP-1-neutralizing antibody or keratinocyte chemoattractant (KC)-neutralizing antibody (NAb or ab; 8 μg/mouse, iv) were administered 60 minutes after injection of lipopolysaccharide (LPS, 0.5 mg/kg, iv). Mononuclear cells in the myocardium were detected by immunofluorescence staining, and levels of TNF-α, IL-1β, and IL-6 in the myocardium and plasma were analyzed by ELISA 6 h after LPS treatment. Neutralization of MCP-1, not KC, reduced myocardial mononuclear cell density (A) and cytokine levels in the myocardium and plasma (B and C). Greater reductions were observed in old mice. Data are expressed as mean ± standard error. *P <0.05 versus corresponding control (Ctrl); ^#P <0.05 versus adult mice treated with LPS alone or LPS + KC-NAb; †P <0.05 versus corresponding age group treated with LPS alone or LPS + KC-Nab.