Figure 2
From: Silencing airway epithelial cell-derived hepcidin exacerbates sepsis-induced acute lung injury
Evaluation of hepcidin silencing in vivo after cecal ligation and puncture (CLP) surgery. (A, B) Hepcidin expression in lungs was detected by quantitative polymerase chain reaction and immunohistochemistry. A representative image is presented (magnification: ×400), and quantified data are shown. n = 5 mice/group. *P <0.001. (C, D) Hepcidin mRNA and protein levels in alveolar macrophages were measured using quantitative polymerase chain reaction and immunohistochemistry. A representative image is presented (magnification: ×400), and quantified data are shown. n = 4 to 5 mice/group. (E, F) Hepcidin expression in livers was examined by quantitative polymerase chain reaction and immunohistochemistry. A representative image is presented (magnification: ×400), and quantified data are shown. n = 5 mice/group.