Figure 3From: Lack of effect of glutamine administration to boost the innate immune system response in trauma patients in the intensive care unitConcentration of TNFα in cell culture supernatants in trauma patients treated with and without glutamine. TLR functionality. Levels of TNFα analyzed by a bead array ELISA (CBA Kit, BD Biosciences), in response to lipopolysaccharide (LPS-100 ng/mL), Pam3CSK4 (PAM-10 pg/mL) and zymosan (ZYM-10 pg/mL) at the beginning of the treatment (Figure 3A); at Day 6 (Figure 3B) and at Day 14 (Figure 3C). Monocytes from trauma patients treated with glutamine subjects (black bars, n = 23) and trauma patients without glutamine (white bars, n = 20). Control bars are samples production of cytokines by unstimulated monocytes. Data are given as mean ± SEM.Back to article page