Mechanisms of leukocyte distribution during sepsis: an experimental study on the interdependence of cell activation, shear stress and endothelial injury

Table 1 Description of the different groups and their activation protocol

Group	Activation	Description
HUVEC-/PMN-	HUVEC 0 ng/ml LPS	Control (non-inflamed tissue)
	+ PMN 0 ng/ml LPS
HUVEC++/PMN-	HUVEC 100 ng/ml LPS	Maximal local inflammation
	+ PMN 0 ng/ml LPS
HUVEC++/PMN ++	HUVEC 100 ng/ml LPS	Maximal systemical inflammation in the hyper-inflammatory stage of sepsis
	+ PMN 100 ng/ml LPS
HUVEC+/PMN-	HUVEC 10 ng/ml LPS	Submaximal local inflammation
	+ PMN 0 ng/ml LPS
HUVEC+/PMN +	HUVEC 10 ng/ml LPS	Submaximal systemical inflammation in the hypo-inflammatory stage of sepsis
	+ PMN 10 ng/ml LPS
HUVEC++/PMN++/PLT	HUVEC 100 ng/ml LPS	Maximal systemical inflammation and endothelial damage in the hyper-inflammatory stage of sepsis
	+ PMN 100 ng/ml LPS
HUVEC+/PMN+/PLT	HUVEC 10 ng/ml LPS	Submaximal systemical inflammation and endothelial damage in the hypo-inflammatory stage of sepsis
	+ PMN 10 ng/ml LPS

HUVEC, human umbilical venous endothelial cells; PMN, polymorphonuclear neutrophils; PLT, platelets; LPS, lipopolysaccharide.

ISSN: 1364-8535