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Figure 5 | Critical Care

Figure 5

From: Inhibition of monocyte chemoattractant protein-1 prevents diaphragmatic inflammation and maintains contractile function during endotoxemia

Figure 5

Effects of MCP-1 treatment on inflammatory markers in cultured skeletal muscle cells. (a) Representative RNase protection assays. (b) Quantification of MCP-1 mRNA levels, after in vitro stimulation of primary diaphragmatic myotube cultures with recombinant MCP-1 (100 ng/ml). (c) NF-κB transcriptional activity in C2C12 myotube cultures treated with recombinant MCP-1 (100 ng/ml), as determined by the plasmid transfection luciferase reporter system. *P < 0.05 for vehicle-versus MCP-1-treated groups.

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Critical Care

ISSN: 1364-8535