Figure 5

Renal function and urine, serum, and renal IL-6 in cisplatin-induced AKI. (A) Serum creatinine and (B) BUN increase on Day 3 in mice with cisplatin-induced AKI. Serum creatinine and BUN were determined on Days 1, 2 and 3 after vehicle or cisplatin injection and was significantly increased on Day 3 (P < 0.05 for creatinine and P = 0.0001 for BUN vs. Veh; n = 5 to 12). (C) Urine IL-6 increases on Days 2 and 3 in mice with cisplatin-induced AKI. Urine was collected at the time of sacrifice on Days 1, 2 and 3 after vehicle or cisplatin injection. Urine IL-6 did not increase significantly until Days 2 and 3 after cisplatin injection, when proximal tubule injury is present (P < 0.002 vs. Veh, n = 5 to 12). (D) Serum IL-6 increases on Days 2 and 3 in mice with cisplatin-induced AKI. Serum IL-6 was determined on Days 1, 2 and 3 after vehicle or cisplatin injection and was significantly increased on Days 2 and 3 (P < 0.05 on Day 2 and P < 0.0001 on Day 3 vs. Veh; n = 5 to 12). (E) Kidney IL-6 increases on Day 3 in mice with cisplatin-induced AKI. Kidney IL-6 was determined on Days 1, 2 and 3 after vehicle or cisplatin injection and was significantly increased on Day 3 (P < 0.01 on Day 3 vs. Veh; n = 5 to 12).