Figure 4

Delayed argon application. In this setting, groups were incubated for 72 hours in an atmosphere of 50% Argon, 21% O₂, 5% CO₂ and 24% N₂, either directly after trauma was induced (t = 0) or with a two or three hours delay. All groups were normalized to their respective trauma control group at t = 72 hours. Panel A shows the results for OGD and panel B the results for TBI. In the OGD group an average of 43 slices with a minimum of 22 slices was used (prepared from three mice per group). We found a significant difference between the control group and each tested time point (*P ≤ 0.001). Moreover the trauma intensity between t = 0 hours and t = 3 hours differed significantly (P ≤ 0.05). In the TBI group the detected trauma after zero, two and three hours delay time was significantly lower compared to the trauma control group (*P ≤ 0.001). Furthermore, trauma intensity after three-hour delay time was significantly increased as compared to zero and two-hour delay (P ≤ 0.001). An average of 31 slices and a minimum of 15 slices was used for each group (prepared from two to three mice).