Volume 1 Supplement 1

17th International Symposium on Intensive Care and Emergency Medicine

Open Access

Twenty-nine day study of stability for six different parenteral nutrition mixtures

  • JC Desport1, 2,
  • B Hoedt3,
  • V Pelagatti3,
  • A Lagarde3,
  • F Lorieul4,
  • D Chalmel4,
  • F Sultan4 and
  • P Feiss1
Critical Care19971(Suppl 1):P098

https://doi.org/10.1186/cc82

Published: 1 March 1997

Aim of the study

Parenteral nutrition is often peri-operative. It can be realized with packages containing different nutrients. Knowledge of the stability of these mixtures is necessary. The aim of this study is to test the stability of six parenteral nutrition mixtures, fitted to different pathologies.

Materials and methods

The mixtures are not supplemented. The packages are made of EVA (Nutripoches NPP235; Pharmacia). They contain (Table) glucose (G50 or G30%: Aguettant and Cooper Lab), amino acids (Hyperamine 25® B Braun Medical SA), and lipids consisting of medium chain and long chain triglycerides (20% Médialipide® B Braun Medical SA) or only long chain (20% Intralipide® Pharmacia). The Central Pharmacy of the hospital fills the packages in a clean room under a laminary horizontal flux hood. The packages are filled with nutrients under nitrogen pressure. Two packages of each type are forwarded by air freight in a cold box to the stability control laboratory within 24 h after manufacture. They are stored in a refrigerator at 4 ± 1°C (circulating air) during 28 days, then 24 h at room temperature. The stability tests are performed at Dl, D3, D7, D10, D14, D21, D28, D28 + 24 h. The tests are: visual macroscopic (with and without homogenization) and microscopic controls (dilution = 1 : 10, increase: × 500 and × 1000), granulometric analysis (Coulter Counter TA II with a 50 μm aperture tube, dilutions = 1 : 100,000 and 1 : 1000), controls of pH, osmolality and conformity to the formulae. Results are given compared to mother emulsions.

Results

There is no macroscopic destabilization from D1 to D29 (no change in the colour or aspect of the mixtures). They are microscopically identical with the D1 mother emulsions. There is neither granulometric destabilization nor break of the emulsion incompatible with a normal therapeutic use. Conformity to the formulae, osmolality and pH are not significantly different from D1 to D29.

Conclusion

All the stability tests comply: the controlled mixtures are stable during a long storage period: 28 days at 4°C temperature, plus 24 h at room temperature.
Table:

Contents of the six parenteral nutritions

 

1

2

3

4

5

6

G 50% (ml)

  

500

500

650

600

G 30% (ml)

750

750

    

IL 20% (ml)

500

 

500

   

M 20% (ml)

 

500

 

500

500

600

H 25 (ml)

500

500

500

500

750

750

N (g)

12.8

12.8

12.8

12.8

19.2

19.2

G/L (%)

47/53

48/52

50/50

50/50

57/43

50/50

C/N

148

147

156

155

119

124

Kcal G + L

1900

1884

2000

1984

2284

2381

Authors’ Affiliations

(1)
Anesthésie Réanimation Chirurgicale et
(2)
Unité Fonctionnelle de Nutrition
(3)
Pharmacie Centrale
(4)
B Braun Medical SA, Av Mal Juin

Copyright

© Current Science Ltd 1997

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