3,4-Methylenedioxyphenol attenuates systemic inflammation and oxidative stress in septic rats

Introduction

Sepsis is one of the major causes of mortality in ICUs. Systemic inflammation and oxidative stress are involved in the pathogenesis and development of sepsis. 3,4-Methylenedioxyphenol (sesamol), one of the lignans in sesame oil, protects against endotoxin-induced oxidative stress and organ failure. However, the effects of sesamol on systemic inflammation and oxidative stress in septic rats have never been investigated.

Objective

To investigate the effects of sesamol on systemic inflammation and oxidative stress in septic rats.

Methods

Septic rats were induced by cecal ligation and puncture (CLP). Rats received sesamol (10 mg/kg, subcutaneously) 0 and 6 hours after CLP. IL-1β, lipid peroxidation, hydroxyl radical, superoxide anion, xanthine oxidase activity, and nitrite levels in blood were determined 12 hours after CLP.

Results

IL-1β, lipid peroxidation, hydroxyl radical, superoxide anion, xanthine oxidase activity, and nitrite levels were significantly increased in CLP-treated rats compared with those in the sham-operation group (all P < 0.05). Sesamol significantly reduced IL-1β, lipid peroxidation, hydroxyl radical, superoxide anion, xanthine oxidase activity, and nitrite levels compared with the saline group in CLP-treated rats (all P < 0.05).

Conclusion

Sesamol might attenuate systemic inflammation and oxidative stress by inhibiting proinflammatory cytokine and reactive oxygen species generation in septic rats.