Volume 13 Supplement 4

Sepsis 2009

Open Access

Muscimol increases the survival rate and inhibits the inflammatory response in endotoxemic mice

  • D-Z Hsu1,
  • Y-H Li1,
  • P-Y Chu1 and
  • M-Y Liu1
Critical Care200913(Suppl 4):P19

https://doi.org/10.1186/cc8075

Published: 11 November 2009

Introduction

Affecting the γ-amino butyric acid (GABA) pathway results in an alteration of inflammatory response in various animal models. However, its mechanism is still unclear. The aim of this study was to determine the effects of muscimol, a GABAA receptor agonist, on lipopolysaccharide-induced mortality and inflammation in mice.

Materials

C57BL6 mice, lipopolysaccharide (derived from Escherichia coli, serotype O55:B5), and muscimol were used in this study.

Methods

Mice endotoxemia was induced by 10 mg/kg lipopolysaccharide intraperitoneally. Muscimol ranging from 0 to 3 mg/kg were given subcutaneously 30 minutes before lipopolysaccharide administration. Serum TNFα, IL-1β, IL-10, and IL-12 were determined using ELISA.

Results

Muscimol significantly increased the survival rate in sub-lethal dose of lipopolysaccharide-treated mice (from 7% to 100%) (P < 0.0001) within 72 hours. Muscimol inhibited serum TNFα, IL-1β, and IL-12 production in a dose-dependent manner. Furthermore, muscimol significantly increased serum IL-10 levels (P < 0.001) in lipopolysaccharide-treated mice.

Conclusion

Muscimol potently increased the survival rate and inhibited inflammatory response in endotoxemic mice.

Authors’ Affiliations

(1)
Department of Environmental and Occupational Health, National Cheng Kung University Medical College

Copyright

© BioMed Central Ltd 2009

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