Intravenous glutamine decreases lung and distal organ injury in an experimental model of abdominal sepsis

Table 2 Semi-quantitative analysis of lung and diaphragm electron microscopy

Groups	Type II epithelial cell lesion	Hyaline membrane	Endothelial cell damage	Oedema of Z-disc	Diaphragm mitochondrial injury
C-SAL	0 (0 to 1)	0 (0 to 0)	0 (0 to 0)	0 (0 to 0)	0 (0 to 0)
C-Gln	0 (0 to 0.25)	0 (0 to 0)	0 (0 to 0)	0 (0 to 0)	0 (0 to 0)
CLP-SAL	3 (2 to 3)*	2 (2 to 3)*	4 (3 to 4)*	3 (2 to 4)*	3 (2 to 3)*
CLP-Gln	1 (1 to 2)* #	0 (0 to 1)	1 (1 to 2)* #	0 (0 to 1)	1 (0 to 1)* #

Values are median (25^th to 75^th percentile) of five rats in each group. The pathologic findings were graded according to a five-point semi-quantitative severity-based scoring system: 0 = normal lung parenchyma or diaphragm, 1 = changes in 1 to 25%, 2 = changes in 26 to 50%, 3 = changes in 51 to 75%, and 4 = changes in 76 to 100% of the examined tissue. Sepsis was induced by cecal ligation and puncture surgery (CLP). A sham-operated group was used as control (C) for animals undergoing CLP. One hour after surgery, C and CLP groups were treated with saline (SAL) or glutamine (Gln). *Significantly different from C group (P < 0.05). #Significantly different from CLP-SAL group (P < 0.05).

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