Figure 6

Representative photomicrographs of kidney, liver and small intestine villi stained with (upper panels) haematoxylin & eosin and (lower panels) immunohistochemical staining for FasL. (Kidney). Control (C) group shows glomeruli (G) and renal tubules (T) with preserved architecture and sparse apoptotic renal cells (arrowheads). Cecal ligation and puncture (CLP) group presents disarrangement of renal tubules with degenerative cytoplasmic changes (arrows) and numerous apoptotic cells. Note in CLP group treated with glutamine (Gln) that the histoarchitecture of the renal tubules is restored with a decrease in apoptotic cells (arrowheads). (Liver) C group shows hepatocytes (H) adjacent to centro-lobular vein (CLV) with preserved architecture and few apoptotic cells. In CLP group treated with saline (SAL). CLP-SAL group shows disarrangement of hepatocytes with diffuse microvacuolization by fat degeneration (arrows) and numerous apoptotic cells. Note that in CLP group treated with Gln, the histoarchitecture of the hepatocytes is restored with decreased apoptotic cells (arrowheads). (Small intestine villi) C group depicted preserved architecture with normal crypts (Cry) and villi (Vil) with few apoptotic cells. CLP presents necrosis of the top of villi (Nec), degenerative cytoplasmic changes of enterocytes (arrows), and numerous apoptotic cells. In CLP-Gln group, the histoarchitecture of the crypts and villi is restored with decrease of the apoptotic cells (arrowheads).