Time course of Ang-2, cytokines, and adhesion molecules after LPS challenge in healthy subjects. (a) Concentrations of circulating angiopoietin (Ang)-2 compared with plasma levels of TNF-alpha. IL-6, IL-8, and C-reactive protein (CRP) after lipopolysaccharide (LPS) challenge in six healthy volunteers. (b) Concentrations of circulating Ang-2 compared with plasma levels of endothelial adhesion molecules E-selectin, P-selectin, and inter-cellular adhesion molecule-1 (ICAM-1) after LPS challenge in six healthy volunteers. Non-parametric analysis of variance (Friedman's test) with Dunn's test for multiple comparison (two-sided) was used to demonstrate statistical changes in Ang-2, cytokines, and adhesion molecules (y-axes denote percentage increase; baseline = 100%).