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  • Meeting abstract
  • Open Access

Interleukin-17 stimulates intraperitoneal neutrophil infiltration through the release of the chemokine GROα from peritoneal mesothelial cells

  • 1, 2,
  • 2,
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  • 1,
  • 2,
  • 2,
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  • 3,
  • 1,
  • 1 and
  • 1
Critical Care20004 (Suppl 1) :P60

https://doi.org/10.1186/cc780

  • Published:

Keywords

  • Peritoneal Cavity
  • Neutrophil Infiltration
  • Leukocyte Infiltration
  • Leukocyte Recruitment
  • Neutrophil Accumulation

Full text

Introduction

IL-17 is a newly discovered cytokine implicated in the regulation of hematopoiesis and inflammation. Since IL-17 production is restricted to activated T lymphocytes the effects exerted by IL-17 may help to understand the contribution of T cells to the inflammatory response. We investigated the role of IL-17 in leukocyte recruitment into the peritoneal cavity.

Methods

Leukocyte infiltration in vivo was assessed in BalB/ CJ mice. Effects of IL-17 on chemokine generation in vitro were examined in human peritoneal mesothelial cells (HPMC).

Results

Intraperitoneal administration of IL-17 resulted in a selective recruitment of neutrophils into the peritoneum and increased levels of KC chemokine (murine homologue of human GROα). Pre-treatment with anti-KC antibody significantly reduced the IL-17-driven neutrophil accumulation. Primary cultures of HPMC expressed IL-17 receptor mRNA. Exposure of HPMC to IL-17 led to a dose- and time-dependent induction of GROα mRNA and protein. Combination of IL-17 together with TNFα resulted in an increased stability of GROα mRNA and synergistic release of GROα protein. Anti-IL-17 antibody blocked the effects of IL-17 in vitro and in vivo.

Conclusions

IL-17 is capable of selectively recruiting neutrophils into the peritoneal cavity via the release of neutrophil-specific chemokines from the peritoneal mesothelium.

Authors’ Affiliations

(1)
Nephrology and Medical Intensive Care, UK Charité, Campus Virchow-Klinikum, Augustenburger Platz 1, Berlin, D-13353, Germany
(2)
Pathophysiology, University Medical School, Poznan, Poland
(3)
Visceral and Transplant Surgery, University of Bern, Inselspital, Bern, Switzerland

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