Figure 4
From: Central sympatholytics prolong survival in experimental sepsis
Whole blood incubation with lipopolysaccharide and clonidine respectively. Whole blood samples of healthy donors (n = 4) were incubated for 24 hours with either LPS alone (1 μg) or LPS (1 μg) plus clonidine (10-4). (a) IL-1β levels after whole blood incubation with LPS and clonidine respectively. The concentration of IL-1β increased significantly after LPS administration compared with untreated controls (p = 0.0005). There was no statistically significant difference in IL-1β levels between samples treated with LPS alone and those incubated with LPS plus clonidine (10-4; p = 0.89). (b) IL-6 levels after whole blood incubation with LPS and clonidine respectively. The concentration of IL-6 increased significantly after LPS administration compared with untreated controls (p = 0.0001). There was no statistically significant difference in IL-6 levels between samples treated with LPS alone and those incubated with LPS plus clonidine (10-4; p = 0.88). (c) TNF-α levels after whole blood incubation with LPS and clonidine respectively. The concentration of TNF-α increased significantly after LPS administration compared with untreated controls (p = 0.025). There was no statistically significant difference in TNF-α levels between samples treated with LPS alone and those incubated with LPS plus clonidine (10-4; p = 0.99). * indicates statistical significant differences when compared with untreated.