Figure 3

Clonidine reduces nuclear factor (NF) κB activation after caecal ligation and puncture (CLP)-induced sepsis. The figure shows NF-κB binding activity, as assessed by electrophoretic mobility shift assay (EMSA) assay in the livers of mice that underwent CLP surgery. CLP led to a strong increase in NF-κB binding activity. In contrast, in mice treated with clonidine we found no difference in NF-κB binding activity compared with mice receiving placebo injections with normal saline. Furthermore, there was no difference between mice with CLP-induced sepsis receiving clonidine and sham-operated mice. Group I 'Sham OP'; EMSA for NF-κB binding activity; liver tissue. Group II 'CLP + clonidine', clonidine therapy, EMSA for NF-κB binding activity; liver tissue. Group III: 'CLP + placebo', saline injection, EMSA for NF-κB binding activity; liver tissue