- Poster presentation
- Open Access
Cytochrome C is not released in the heart during sepsis-induced myocardial depression
Critical Care volume 13, Article number: P369 (2009)
Sepsis is often accompanied by myocardial depression; one of the possible mechanisms includes mitochondrial injury. We hypothesized that opening of the mitochondrial permeability transition pore (mPTP) may occur during sepsis-induced myocardial depression. We investigated the opening of the mPTP in septic hearts by measuring cytochrome C release into the cytosol.
Sepsis was induced in rats by 7.5 mg/kg lipopolysaccharide injection intraperitoneally. After 4 hours, hearts were excised and mounted in a Langendorff setup to study myocardial contractility ex vivo. Subsequently, hearts were frozen and 5 μm cryostat sections were made. Sections were stained for cytochrome C using immunohistochemistry. Healthy rats served as controls.
Septic animals showed a decreased contractility (P < 0.005) and lower developed pressure (P < 0.001) when compared with healthy controls. Immunohistochemistry revealed no release of cytochrome C in healthy or septic hearts.
Cytochrome C is not released during sepsis-induced myocardial depression. These results indicate that the mitochondrial permeability pore may not be involved in the development of myocardial dysfunction during sepsis.
About this article
Cite this article
Smeding, L., Van Veelen, T., Laarse, W.V.d. et al. Cytochrome C is not released in the heart during sepsis-induced myocardial depression. Crit Care 13, P369 (2009). https://doi.org/10.1186/cc7533
- Public Health
- Emergency Medicine
- Mitochondrial Permeability Transition
- Myocardial Contractility