Skip to main content

Advertisement

We’d like to understand how you use our websites in order to improve them. Register your interest.

Cytochrome C is not released in the heart during sepsis-induced myocardial depression

Introduction

Sepsis is often accompanied by myocardial depression; one of the possible mechanisms includes mitochondrial injury. We hypothesized that opening of the mitochondrial permeability transition pore (mPTP) may occur during sepsis-induced myocardial depression. We investigated the opening of the mPTP in septic hearts by measuring cytochrome C release into the cytosol.

Methods

Sepsis was induced in rats by 7.5 mg/kg lipopolysaccharide injection intraperitoneally. After 4 hours, hearts were excised and mounted in a Langendorff setup to study myocardial contractility ex vivo. Subsequently, hearts were frozen and 5 μm cryostat sections were made. Sections were stained for cytochrome C using immunohistochemistry. Healthy rats served as controls.

Results

Septic animals showed a decreased contractility (P < 0.005) and lower developed pressure (P < 0.001) when compared with healthy controls. Immunohistochemistry revealed no release of cytochrome C in healthy or septic hearts.

Conclusion

Cytochrome C is not released during sepsis-induced myocardial depression. These results indicate that the mitochondrial permeability pore may not be involved in the development of myocardial dysfunction during sepsis.

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Smeding, L., Van Veelen, T., Laarse, W.V.d. et al. Cytochrome C is not released in the heart during sepsis-induced myocardial depression. Crit Care 13, P369 (2009). https://doi.org/10.1186/cc7533

Download citation

Keywords

  • Public Health
  • Cytosol
  • Emergency Medicine
  • Mitochondrial Permeability Transition
  • Myocardial Contractility